Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp196 | Cell biology: osteoblasts and bone formation | ECTS2013

The effect of enamel matrix derivative on human gingival fibroblasts cultured on zirconium disc surfaces

Lee Heesu , Pae Ahran , Kwon Yong-Dae , Ko Seonghee

Purpose: To investigate the effect of enamel matrix derivative (Emdogain) on the attachment, growth behavior and the genetic effect of human gingival fibroblasts (HGF) cultured on zirconium disc surfaces.Materials and methods: HGF cells were cultured on i) zirconium discs without enamel matrix derivative (EMD), ii) zirconium discs with EMD 25 μg/ml, and iii) zirconium discs with EMD 100 μg/ml. The cell proliferation activity was evaluated throu...

ba0003pp142 | Cell biology: osteoblasts and bone formation | ECTS2014

Cell attachment and proliferation of bone marrow-derived osteoblast on zirconia of various surface treatment

Lee Heesu , Pae Ahran , Noh Kwantae , Woo Yi-Hyung

This study was performed to characterize the effects of calcium phosphate coated and hydroxyapatite coated zirconia compared to smooth surfaced zirconia with bone marrow-derived osteoblast culture.Bone marrow-derived osteoblasts were cultured on i) smooth zirconia, ii) zirconia coated with calcium phosphate (CaP), and iii) zirconia coated with hydroxyapatite (HA). The tetrazolium-based colorimetric assay (MTT test) was used for examining the attachment o...